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Swant rabbit anti calbindin antibody
Rabbit Anti Calbindin Antibody, supplied by Swant, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-calbindin/anti+calbindin/pm42119675-127-30-41
Average 86 stars, based on 1 article reviews
rabbit anti calbindin antibody - by Bioz Stars, 2026-09
86/100 stars

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Incubation:

Article Title: Capillary connections between sensory circumventricular organs and adjacent parenchyma enable local volume transmission.
Article Snippet: A mixture of rabbit anti-calretinin (1:500, Chemicon, AB149, LOT 25797041, Temecula, CA) and rabbit anti-calbindin (1:5000, Swant, CB38a, Burgdorf, Switzerland LOT 9.03) to identify hypothalamic and brainstem regions adjacent to the SFO and AP.38,39 Secondary antibodies were as follows: for calretinin and calbindin, donkey anti-rabbit Cy2 (1:200, Jackson ImmunoResearch); for SMA, donkey anti-mouse Cy3 (1:200, Jackson ImmunoResearch); for collagen, donkey anti-goat Cy5 (1:200, Jackson ImmunoResearch).

Article Title: Cerebellar Purkinje cell stripe patterns reveal a differential vulnerability and resistance to cell loss during normal aging in mice
Article Snippet: Rabbit anti-calbindin (1:10,000; Swant) or mouse anti-calbindin (1:2,000; Sigma) was used to label all Purkinje cells.

Article Title: Reward expectation and receipt differentially modulate the spiking of accumbens D1+ and D2+ neurons.
Article Snippet: The sections were incubated overnight at room temperature in a primary antibody solution comprised of 1:1000 rabbit anti-calbindin (Swant), 1:500 mouse anti-CD11b (a marker for microglia, Bio-Rad), 1% normal goat serum, and 0.1% Triton-X 100 in PBS.

Article Title: Viral gene therapy as treatment for cholesterol storage disease or disorder
Article Snippet: Sections were incubated overnight at 4° C. with either rabbit anti-calbindin (1:3000, Swant), rabbit anti-Npc1 (1:2000), or mouse anti-NeuN (1:1000, MILIPORE) in PBSt, and the primaries detected using DyLight-488 goat anti-rabbit/mouse IgG or Alexa-594 anti-rabbit (1:1000 in PBSt, Vector Labs).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In MafB-V1 mice we amplified both EGFP and tdT as above and combined with the following antibodies: rabbit anti-calbindin (Swant), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), and goat anti-Sp8 (Santa Cruz).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In birthdating experiments we used in serial sections either rabbit anti-MafB (Sigma), goat anti-Foxp2 (Sant Cruz), rabbit anti-Pou6f2 (Sigma), goat anti-Sp8 (Santa Cruz), guinea pig anti-Otp (Jessell Lab; ), rabbit anti-Otp (Jessell Lab; ), rabbit anti-Foxp4 (Jessell Lab; ), or rabbit anti-calbindin (Swant).

Article Title: Hippocampal area CA2 activity supports social investigation following an acute social stress
Article Snippet: A subset of sections was stained for calbindin+ cells (10 rabbit anti-calbindin (Swant #CB38) 1:500 + 20 anti-rabbit-633 (Invitrogen #A21071) 1:500).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: To analyze Otp-V1 interneurons, EGFP fluorescence was amplified with chicken anti-EGFP antibodies as above, and one of the following additional primary antibodies was added in serial sections: rabbit anti-calbindin (Swant), guinea pig anti-Otp (Jessell Lab, CU), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), or goat anti-Sp8 (Santa Cruz).

Marker:

Article Title: Capillary connections between sensory circumventricular organs and adjacent parenchyma enable local volume transmission.
Article Snippet: A mixture of rabbit anti-calretinin (1:500, Chemicon, AB149, LOT 25797041, Temecula, CA) and rabbit anti-calbindin (1:5000, Swant, CB38a, Burgdorf, Switzerland LOT 9.03) to identify hypothalamic and brainstem regions adjacent to the SFO and AP.38,39 Secondary antibodies were as follows: for calretinin and calbindin, donkey anti-rabbit Cy2 (1:200, Jackson ImmunoResearch); for SMA, donkey anti-mouse Cy3 (1:200, Jackson ImmunoResearch); for collagen, donkey anti-goat Cy5 (1:200, Jackson ImmunoResearch).

Article Title: Cerebellar Purkinje cell stripe patterns reveal a differential vulnerability and resistance to cell loss during normal aging in mice
Article Snippet: Rabbit anti-calbindin (1:10,000; Swant) or mouse anti-calbindin (1:2,000; Sigma) was used to label all Purkinje cells.

Article Title: Reward expectation and receipt differentially modulate the spiking of accumbens D1+ and D2+ neurons.
Article Snippet: The sections were incubated overnight at room temperature in a primary antibody solution comprised of 1:1000 rabbit anti-calbindin (Swant), 1:500 mouse anti-CD11b (a marker for microglia, Bio-Rad), 1% normal goat serum, and 0.1% Triton-X 100 in PBS.

Article Title: Viral gene therapy as treatment for cholesterol storage disease or disorder
Article Snippet: Sections were incubated overnight at 4° C. with either rabbit anti-calbindin (1:3000, Swant), rabbit anti-Npc1 (1:2000), or mouse anti-NeuN (1:1000, MILIPORE) in PBSt, and the primaries detected using DyLight-488 goat anti-rabbit/mouse IgG or Alexa-594 anti-rabbit (1:1000 in PBSt, Vector Labs).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In MafB-V1 mice we amplified both EGFP and tdT as above and combined with the following antibodies: rabbit anti-calbindin (Swant), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), and goat anti-Sp8 (Santa Cruz).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In birthdating experiments we used in serial sections either rabbit anti-MafB (Sigma), goat anti-Foxp2 (Sant Cruz), rabbit anti-Pou6f2 (Sigma), goat anti-Sp8 (Santa Cruz), guinea pig anti-Otp (Jessell Lab; ), rabbit anti-Otp (Jessell Lab; ), rabbit anti-Foxp4 (Jessell Lab; ), or rabbit anti-calbindin (Swant).

Article Title: Hippocampal area CA2 activity supports social investigation following an acute social stress
Article Snippet: A subset of sections was stained for calbindin+ cells (10 rabbit anti-calbindin (Swant #CB38) 1:500 + 20 anti-rabbit-633 (Invitrogen #A21071) 1:500).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: To analyze Otp-V1 interneurons, EGFP fluorescence was amplified with chicken anti-EGFP antibodies as above, and one of the following additional primary antibodies was added in serial sections: rabbit anti-calbindin (Swant), guinea pig anti-Otp (Jessell Lab, CU), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), or goat anti-Sp8 (Santa Cruz).

Fluorescence:

Article Title: Capillary connections between sensory circumventricular organs and adjacent parenchyma enable local volume transmission.
Article Snippet: A mixture of rabbit anti-calretinin (1:500, Chemicon, AB149, LOT 25797041, Temecula, CA) and rabbit anti-calbindin (1:5000, Swant, CB38a, Burgdorf, Switzerland LOT 9.03) to identify hypothalamic and brainstem regions adjacent to the SFO and AP.38,39 Secondary antibodies were as follows: for calretinin and calbindin, donkey anti-rabbit Cy2 (1:200, Jackson ImmunoResearch); for SMA, donkey anti-mouse Cy3 (1:200, Jackson ImmunoResearch); for collagen, donkey anti-goat Cy5 (1:200, Jackson ImmunoResearch).

Article Title: Cerebellar Purkinje cell stripe patterns reveal a differential vulnerability and resistance to cell loss during normal aging in mice
Article Snippet: Rabbit anti-calbindin (1:10,000; Swant) or mouse anti-calbindin (1:2,000; Sigma) was used to label all Purkinje cells.

Article Title: Reward expectation and receipt differentially modulate the spiking of accumbens D1+ and D2+ neurons.
Article Snippet: The sections were incubated overnight at room temperature in a primary antibody solution comprised of 1:1000 rabbit anti-calbindin (Swant), 1:500 mouse anti-CD11b (a marker for microglia, Bio-Rad), 1% normal goat serum, and 0.1% Triton-X 100 in PBS.

Article Title: Viral gene therapy as treatment for cholesterol storage disease or disorder
Article Snippet: Sections were incubated overnight at 4° C. with either rabbit anti-calbindin (1:3000, Swant), rabbit anti-Npc1 (1:2000), or mouse anti-NeuN (1:1000, MILIPORE) in PBSt, and the primaries detected using DyLight-488 goat anti-rabbit/mouse IgG or Alexa-594 anti-rabbit (1:1000 in PBSt, Vector Labs).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In MafB-V1 mice we amplified both EGFP and tdT as above and combined with the following antibodies: rabbit anti-calbindin (Swant), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), and goat anti-Sp8 (Santa Cruz).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In birthdating experiments we used in serial sections either rabbit anti-MafB (Sigma), goat anti-Foxp2 (Sant Cruz), rabbit anti-Pou6f2 (Sigma), goat anti-Sp8 (Santa Cruz), guinea pig anti-Otp (Jessell Lab; ), rabbit anti-Otp (Jessell Lab; ), rabbit anti-Foxp4 (Jessell Lab; ), or rabbit anti-calbindin (Swant).

Article Title: Hippocampal area CA2 activity supports social investigation following an acute social stress
Article Snippet: A subset of sections was stained for calbindin+ cells (10 rabbit anti-calbindin (Swant #CB38) 1:500 + 20 anti-rabbit-633 (Invitrogen #A21071) 1:500).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: To analyze Otp-V1 interneurons, EGFP fluorescence was amplified with chicken anti-EGFP antibodies as above, and one of the following additional primary antibodies was added in serial sections: rabbit anti-calbindin (Swant), guinea pig anti-Otp (Jessell Lab, CU), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), or goat anti-Sp8 (Santa Cruz).

Amplification:

Article Title: Capillary connections between sensory circumventricular organs and adjacent parenchyma enable local volume transmission.
Article Snippet: A mixture of rabbit anti-calretinin (1:500, Chemicon, AB149, LOT 25797041, Temecula, CA) and rabbit anti-calbindin (1:5000, Swant, CB38a, Burgdorf, Switzerland LOT 9.03) to identify hypothalamic and brainstem regions adjacent to the SFO and AP.38,39 Secondary antibodies were as follows: for calretinin and calbindin, donkey anti-rabbit Cy2 (1:200, Jackson ImmunoResearch); for SMA, donkey anti-mouse Cy3 (1:200, Jackson ImmunoResearch); for collagen, donkey anti-goat Cy5 (1:200, Jackson ImmunoResearch).

Article Title: Cerebellar Purkinje cell stripe patterns reveal a differential vulnerability and resistance to cell loss during normal aging in mice
Article Snippet: Rabbit anti-calbindin (1:10,000; Swant) or mouse anti-calbindin (1:2,000; Sigma) was used to label all Purkinje cells.

Article Title: Reward expectation and receipt differentially modulate the spiking of accumbens D1+ and D2+ neurons.
Article Snippet: The sections were incubated overnight at room temperature in a primary antibody solution comprised of 1:1000 rabbit anti-calbindin (Swant), 1:500 mouse anti-CD11b (a marker for microglia, Bio-Rad), 1% normal goat serum, and 0.1% Triton-X 100 in PBS.

Article Title: Viral gene therapy as treatment for cholesterol storage disease or disorder
Article Snippet: Sections were incubated overnight at 4° C. with either rabbit anti-calbindin (1:3000, Swant), rabbit anti-Npc1 (1:2000), or mouse anti-NeuN (1:1000, MILIPORE) in PBSt, and the primaries detected using DyLight-488 goat anti-rabbit/mouse IgG or Alexa-594 anti-rabbit (1:1000 in PBSt, Vector Labs).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In MafB-V1 mice we amplified both EGFP and tdT as above and combined with the following antibodies: rabbit anti-calbindin (Swant), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), and goat anti-Sp8 (Santa Cruz).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: In birthdating experiments we used in serial sections either rabbit anti-MafB (Sigma), goat anti-Foxp2 (Sant Cruz), rabbit anti-Pou6f2 (Sigma), goat anti-Sp8 (Santa Cruz), guinea pig anti-Otp (Jessell Lab; ), rabbit anti-Otp (Jessell Lab; ), rabbit anti-Foxp4 (Jessell Lab; ), or rabbit anti-calbindin (Swant).

Article Title: Hippocampal area CA2 activity supports social investigation following an acute social stress
Article Snippet: A subset of sections was stained for calbindin+ cells (10 rabbit anti-calbindin (Swant #CB38) 1:500 + 20 anti-rabbit-633 (Invitrogen #A21071) 1:500).

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity
Article Snippet: To analyze Otp-V1 interneurons, EGFP fluorescence was amplified with chicken anti-EGFP antibodies as above, and one of the following additional primary antibodies was added in serial sections: rabbit anti-calbindin (Swant), guinea pig anti-Otp (Jessell Lab, CU), guinea pig anti-Pou6f2 (Jessell Lab, CU), goat anti-Foxp2 (Santa Cruz), or goat anti-Sp8 (Santa Cruz).



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ScRNA-seq reveals damage-induced transcriptomes of the greater epithelial ridge and inner phalangeal cells (A) UMAP showing integration of Epcam + cochlear epithelial cells from P4-P7 control ( Ki67 CreERT2/+ ; R26R tdTomato/+ ) and damage ( Ki67 CreERT2/+ ; Lgr5 DTR/+ ; R26R tdTomato/+ ) cochlea, resulting in 18 individual clusters. (B) UMAPs showing gene expression profiles of medial and lateral GER markers. (C) UMAPs showing cell clusters integrated from control and damage cochlea at individual time points. Arrows indicate cell clusters predominantly or exclusively found in the damaged cochlea. (D) Percent distribution of cells from each time point and condition by cluster. To account for differences in the number of profiled cells in each dataset, percent contribution to each cluster was normalized to the dataset size. Blue asterisk denotes proliferating GER cells, red asterisks denote native and responding DC/IPC populations. Note that cluster 10 (native DC/IPCs) are mainly composed of cells from control cochlea, whereas cluster 8 (responding DC/IPC) is entirely composed of cells from damaged cochlea. (E) UMAPs showing a reduction of Lgr5 + cells in the damaged cochlea, as well as an increase in Ki67-tdTomato + cells in the damaged lateral GER and Fabp3 + responding DC/IPCs. Cluster 17 (proliferating cells) express Mki67 . (F) Overlaid expression of “cell cycle” and “pathways of neurodegeneration—multiple diseases” gene signatures, extracted from KEGG Pathways mmu04110 and mmu05022 using clusterProfiler. Cell cycle gene signature is highly enriched in cluster 17 and moderately enriched in cluster 8. See also . (G) Volcano plots showing differentially expressed genes between damage and control GER and IPhC clusters at all four time points (excluding proliferating cell cluster). (H) Immunostaining showing that Galectin1 and <t>Calb1</t> mark medial GER (white brackets) in all three turns of the control cochlea (P6-7). After damage, domains expressing Galectin1 and Calb1 expand laterally into the central GER. Note that Calb1 also labels hair cells. (I) Crabp1 is expressed in medial GER in all three turns of P4 and P7 control cochlea. At P4 after damage, its expression robustly increased and expanded to the central and lateral GER, before returning to similar levels and spatial pattern as controls at P7. (J) Immunostaining shows that Jag1 expression initially decreases in the lateral GER relative to controls at P4, before upregulation at P7 to more intense levels than controls. (K) Quantification of Crabp1-expressing regions shows a significant increase after damage relative to controls at P7 ( n = 3). Unpaired t tests were used for (K). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Brackets represent GER. IHC = inner hair cell (arrows), OHC = outer hair cell, GER = greater epithelial ridge. Scale bars represent 50 μm.
Anti Calbindin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScRNA-seq reveals damage-induced transcriptomes of the greater epithelial ridge and inner phalangeal cells (A) UMAP showing integration of Epcam + cochlear epithelial cells from P4-P7 control ( Ki67 CreERT2/+ ; R26R tdTomato/+ ) and damage ( Ki67 CreERT2/+ ; Lgr5 DTR/+ ; R26R tdTomato/+ ) cochlea, resulting in 18 individual clusters. (B) UMAPs showing gene expression profiles of medial and lateral GER markers. (C) UMAPs showing cell clusters integrated from control and damage cochlea at individual time points. Arrows indicate cell clusters predominantly or exclusively found in the damaged cochlea. (D) Percent distribution of cells from each time point and condition by cluster. To account for differences in the number of profiled cells in each dataset, percent contribution to each cluster was normalized to the dataset size. Blue asterisk denotes proliferating GER cells, red asterisks denote native and responding DC/IPC populations. Note that cluster 10 (native DC/IPCs) are mainly composed of cells from control cochlea, whereas cluster 8 (responding DC/IPC) is entirely composed of cells from damaged cochlea. (E) UMAPs showing a reduction of Lgr5 + cells in the damaged cochlea, as well as an increase in Ki67-tdTomato + cells in the damaged lateral GER and Fabp3 + responding DC/IPCs. Cluster 17 (proliferating cells) express Mki67 . (F) Overlaid expression of “cell cycle” and “pathways of neurodegeneration—multiple diseases” gene signatures, extracted from KEGG Pathways mmu04110 and mmu05022 using clusterProfiler. Cell cycle gene signature is highly enriched in cluster 17 and moderately enriched in cluster 8. See also . (G) Volcano plots showing differentially expressed genes between damage and control GER and IPhC clusters at all four time points (excluding proliferating cell cluster). (H) Immunostaining showing that Galectin1 and Calb1 mark medial GER (white brackets) in all three turns of the control cochlea (P6-7). After damage, domains expressing Galectin1 and Calb1 expand laterally into the central GER. Note that Calb1 also labels hair cells. (I) Crabp1 is expressed in medial GER in all three turns of P4 and P7 control cochlea. At P4 after damage, its expression robustly increased and expanded to the central and lateral GER, before returning to similar levels and spatial pattern as controls at P7. (J) Immunostaining shows that Jag1 expression initially decreases in the lateral GER relative to controls at P4, before upregulation at P7 to more intense levels than controls. (K) Quantification of Crabp1-expressing regions shows a significant increase after damage relative to controls at P7 ( n = 3). Unpaired t tests were used for (K). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Brackets represent GER. IHC = inner hair cell (arrows), OHC = outer hair cell, GER = greater epithelial ridge. Scale bars represent 50 μm.

Journal: iScience

Article Title: Dual mechanisms of supporting cell regeneration in the neonatal mouse cochlea

doi: 10.1016/j.isci.2026.115113

Figure Lengend Snippet: ScRNA-seq reveals damage-induced transcriptomes of the greater epithelial ridge and inner phalangeal cells (A) UMAP showing integration of Epcam + cochlear epithelial cells from P4-P7 control ( Ki67 CreERT2/+ ; R26R tdTomato/+ ) and damage ( Ki67 CreERT2/+ ; Lgr5 DTR/+ ; R26R tdTomato/+ ) cochlea, resulting in 18 individual clusters. (B) UMAPs showing gene expression profiles of medial and lateral GER markers. (C) UMAPs showing cell clusters integrated from control and damage cochlea at individual time points. Arrows indicate cell clusters predominantly or exclusively found in the damaged cochlea. (D) Percent distribution of cells from each time point and condition by cluster. To account for differences in the number of profiled cells in each dataset, percent contribution to each cluster was normalized to the dataset size. Blue asterisk denotes proliferating GER cells, red asterisks denote native and responding DC/IPC populations. Note that cluster 10 (native DC/IPCs) are mainly composed of cells from control cochlea, whereas cluster 8 (responding DC/IPC) is entirely composed of cells from damaged cochlea. (E) UMAPs showing a reduction of Lgr5 + cells in the damaged cochlea, as well as an increase in Ki67-tdTomato + cells in the damaged lateral GER and Fabp3 + responding DC/IPCs. Cluster 17 (proliferating cells) express Mki67 . (F) Overlaid expression of “cell cycle” and “pathways of neurodegeneration—multiple diseases” gene signatures, extracted from KEGG Pathways mmu04110 and mmu05022 using clusterProfiler. Cell cycle gene signature is highly enriched in cluster 17 and moderately enriched in cluster 8. See also . (G) Volcano plots showing differentially expressed genes between damage and control GER and IPhC clusters at all four time points (excluding proliferating cell cluster). (H) Immunostaining showing that Galectin1 and Calb1 mark medial GER (white brackets) in all three turns of the control cochlea (P6-7). After damage, domains expressing Galectin1 and Calb1 expand laterally into the central GER. Note that Calb1 also labels hair cells. (I) Crabp1 is expressed in medial GER in all three turns of P4 and P7 control cochlea. At P4 after damage, its expression robustly increased and expanded to the central and lateral GER, before returning to similar levels and spatial pattern as controls at P7. (J) Immunostaining shows that Jag1 expression initially decreases in the lateral GER relative to controls at P4, before upregulation at P7 to more intense levels than controls. (K) Quantification of Crabp1-expressing regions shows a significant increase after damage relative to controls at P7 ( n = 3). Unpaired t tests were used for (K). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Brackets represent GER. IHC = inner hair cell (arrows), OHC = outer hair cell, GER = greater epithelial ridge. Scale bars represent 50 μm.

Article Snippet: Rabbit anti-calbindin (Calb1) , Cell Signaling Technologies , Cat#13176: RRID: AB_2687400.

Techniques: Control, Gene Expression, Expressing, Immunostaining